Dec 282018

3 Oct Description: pEGFP-N2 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher. Provide pEGFP-N2 vector/plasmid map, full length sequence, antibiotic resistance, size and other information. pEGFP-N2 encodes a red-shifted variant of wild-type GFP which has been optimized for brighter fluorescence and higher expression in mammalian cells.

Author: Doutaur Dolabar
Country: Malawi
Language: English (Spanish)
Genre: Education
Published (Last): 15 August 2007
Pages: 80
PDF File Size: 1.11 Mb
ePub File Size: 1.57 Mb
ISBN: 258-7-82414-531-1
Downloads: 70030
Price: Free* [*Free Regsitration Required]
Uploader: Aralabar

EGFP has been reported to form dimers Jain et al. Additional product, intellectual property, and restricted use information is available at takarabio. ZsGreen1 has been reported to have better expression than EGFP in lung and muscle tissue and in cultured cells Bell et al.

Pevfp cleavage requires at least two copies of the SacII recognition sequence. Additional product, intellectual property, and restricted use information is available at takarabio. Express a rapidly degraded form of ZsGreen1 in studies that require rapid reporter turnover. Mapping the brain, one cell type at a time Learn about pioneering efforts to map the mammalian brain using single-cell transcriptomics.

Simultaneous, equivalent tet-inducible expression of ZsGreen1 and your protein of interest from the same bidirectional promoter. Sticky ends from pehfp BsaI sites may not be compatible.

Looking for EGFP vectors? Upgrade to AcGFP1 or ZsGreen1 vectors

Sticky ends from different SfiI sites may not be compatible. Speed up your mass spec workflow Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass pegfp n2 workflow at room temperature for downstream protein analysis. Looking for EGFP vectors?

Vectors for expressing and visualizing a protein of interest fused pegp AcGFP1, including a prelinearized vector for simple, one-step In-Fusion cloning. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Sequences flanking EGFP have been converted to a Kozak consensus translation initiation site 6 to further increase the pegfp n2 efficiency in eukaryotic cells.

Related Posts  LEIOMIOMA GASTRICO PDF

Fusions to the N terminus of EGFP retain the fluorescent properties of the native protein allowing the localization of the fusion protein in vivo. Efficient cleavage requires at least pegfp n2 copies of ppegfp PluTI recognition sequence. The 1-base overhangs produced by PflFI may be hard to ligate.

Certain trademarks may not be registered in all jurisdictions. Measure CRE activity with a ZsGreen1 reporter that has a high signal-to-noise ratio and a bright signal. Express a rapidly degraded form of ZsGreen1 in studies that require rapid reporter turnover. Log in to enjoy additional benefits Why sign up for an account?

pEGFP-N2 pEGFP N2载体质粒图谱、序列、价格、抗性、大小等基本信息_生物风载体

The coding sequence of the EGFP gene contains more than silent base changes which correspond to human codon-usage preferences 5. Efficient cleavage requires at pegfo two copies of the SfiI recognition sequence. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

Pegfp n2 brands Takara Clontech Pegfp n2. There peggfp EGFP vector can be transfected into mammalian cells using any standard transfection method.

Looking for EGFP vectors? Upgrade to AcGFP1 or ZsGreen1 vectors

If required, stable transformants can be selected using G pegfp n2. Prolonged incubation with NdeI may lead to removal of additional nucleotides. Sticky ends from different TthI sites may not be compatible.

Sticky ends from different AccI sites may not be compatible. A pegfp n2 analysis of novel fluorescent proteins as reporters for gene transfer studies. We use cookies to improve your browsing experience and provide meaningful content. Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy.

Partner with Takara Bio! Deliver and stably express your gene of interest fused to AcGFP1, in a broad range of dividing cells.

Mapping the brain, one cell type at a time Learn about pioneering efforts to map the mammalian brain using single-cell transcriptomics. For alternative plasmids pefp fluorescent tags, try plasmids from Doug Golenbock’s Lab or plasmids from Vladislav Petfp Lab. Sticky ends from different PflFI sites may not be compatible. All trademarks are the property of Takara Bio Inc.

Related Posts  IRF521 DATASHEET PDF

Use with any Living Colors vector that contains a neomycin resistance cassette to create double-stable cell lines. A comparative analysis of novel fluorescent proteins as reporters for gene transfer studies.

Sequences flanking EGFP have been converted to a Kozak consensus translation initiation site 6 to further increase the translation efficiency in eukaryotic cells. Create an account to get started. An account with takarabio. Measure NFkB activity with a ZsGreen1 reporter that has a high signal-to-noise ratio and a bright signal.

As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is n22 to improving the human condition through biotechnology.

Sticky ends from different RsrII sites may not be compatible. Efficient cleavage requires at least two copies of the RsrII recognition sequence.

质粒载体:pEGFP-N2

The coding pegfp n2 of the EGFP gene pegfp n2 more than silent base changes which correspond to human codon-usage preferences 5. Efficient cleavage requires at least two copies of the XmaI recognition sequence. The inserted gene should include the initiating ATG codon.

This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Deliver and stably express your gene of interest fused to AcGFP1, in a broad range of dividing cells.